Volatilomes of milky mushroom (Calocybe indica P&C) estimated through GCMS/MS

The volatilomes of both fresh and dried samples of milky mushroom (Calocybe indica P&C var. APK2) were characterized with GCMS/MS. The gas chromatogram was performed with the ethanolic extract of the samples. The results revealed the presence of increased levels of 1, 4:3, 6-Dianhydro-α-dglucopyranose (57.77%) in the fresh and oleic acid (56.58%) in the dried fruiting bodies. The other important fatty acid components identified both in fresh and dried milky mushroom samples were octadecenoic acid and hexadecanoic acid, which are known for their specific fatty or cucumber like aroma and flavour. The aroma quality of dried samples differed from that of fresh ones with increased levels of nhexadecenoic acid (peak area 8.46 %) compared to 0.38% in fresh samples. In addition, αD-Glucopyranose (18.91%) and ergosterol (5.5%) have been identified in fresh and dried samples respectively. The presence of increased levels of ergosterol indicates the availability of antioxidants and anticancer biomolecules in milky mushroom, which needs further exploration. The presence of α-DGlucopyranose (trehalose) components reveals the chemo attractive nature of the biopolymers of milky mushroom, which can be utilized to enhance the bioavailability of pharmaceutical or nutraceutical preparations.